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Fig. 3. Abnormal migration in Pax6 mutants is due to non-cell
autonomous defects of migrating cells. (A) Experimental procedures
for the cell transplantation between wild type and mutants using whole-embryo
culture. (B,C) Lateral views of the wild-type (B) and
Pax6 mutant telencephalon (C) after 48 hours in whole-embryo culture.
The Pax6 mutant-derived cells stopped at the PSB (arrows in
B,B'), whereas wild-type-derived cells invaded the ventral part of the
mutant telencephalon (arrowhead in C,C'). (D) Comparison of migratory
distance of the GFP-positive cells in the wild type and Pax6 mutant
telencephalon. The number of GFP-positive cells was calculated at each
distance and quantified as a percentage of total number of migrating cells. In
the Pax6 mutant telencephalon, the number of GFP-positive cells
derived from wild type was significantly increased in the area over 1200 µm
distant from the injection point. Data are presented as percentage of the
labeled cells in each area against the total number of labeled cells
(mean±s.d. of three samples in each group).
**P<0.01. Scale bars: 500 µm in B,C; 50 µm in
B',C'.
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