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Fig. 5. Blockade of EphA/ephrin A interaction induced abnormal migration of
olfactory cortex neurons. (A) Experimental procedures for
EphA/ephrin A signaling blockade. (B,C) Migration patterns of
GFP-positive cells in media containing Fc (B) or EphA3 (C). In Fc-containing
media, the GFP-labeled neurons stopped at the PSB (arrows in B), whereas in
the Eph-A3-Fc-containing media, the GFP-labeled neurons crossed the PSB and
invaded the ventral part of the telencephalon (arrowheads in C). (D)
Comparison of the number of GFP-positive cells in the ventral part of the
telencephalon (the purple area) in Fc- and EphA3-treated telencephalon.
Because in control (Fc treated) samples most of the GFP-positive cells aligned
at 1.0-1.2 mm distant from the dorsal margin of the telencephalon, we marked
this point as `prospective PSB' and compared with the number of GFP-positive
cells ventral of this point. Data are presented as percentage of the labeled
cells in the ventral telencephalon against the total number of labeled cells
(mean±s.d. of four samples in each group).
**P<0.01. r, rostral; c, caudal; d, dorsal; v, ventral.
Scale bar: 500 µm.
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