DEV02306 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Development of the ear in P. marinus. (A-F) Projections of confocal z-stacks of FITC-phalloidin-stained P. marinus ears, showing the actin-rich stereocilia of the sensory hair cells and general ear morphology. (A-C) Lateral views, anterior to left, dorsal to top; (D-F) dorsal views, anterior to left, medial to top. ed, endolymphatic duct; hcs, sensory hair cells. Scale bars: 50 mm.

• Supplemental Figure 2 -

  Fig. S2. Development of the ear in L. planeri. Projections of confocal z-stacks of FITC-phalloidin-stained L. planeri ears, showing the actin-rich stereocilia of the sensory hair cells and general ear morphology. Lateral views, anterior to left, dorsal to top. Stages 25 and 29 are composites of more than one image. ed, endolymphatic duct; hcs, sensory hair cells; g, statoacoustic (VIIIth) ganglion. Scale bar: 50 mm.

• Supplemental Figure 3 -

  Fig. S3. Comparison of our P. marinus follistatin amino acid sequences with those of gnathostome vertebrates. This shows the insertion that is present in only the smaller of the two P. marinus clones and in zebrafish follistatin. This insert is also found in follistatin genes of other species. Completely conserved residues are shaded black; identical residues, dark grey; similar residues, light grey; different residues, white.

• Supplemental Figure 4 -

  Fig. S4. Comparison of P. marinus Patched (Ptc) amino acid sequence with Ptc, Ptc1 and Ptc2 of other species. The CLUSTALW dendrogram shown in Fig. S5 in the supplementary material is based on this lineup. zeb, Danio rerio; dros, Drosophila melanogaster; xen, Xenopus laevis; chick, Gallus gallus; mouse, Mus musculus. Completely conserved residues are shaded black; identical residues, dark grey; similar residues, light grey; different residues, white.

• Supplemental Figure 5 -

  Fig. S5. Unrooted phylogenetic tree predicted by CLUSTALW analysis of amino acid sequence, showing the relationship of P. marinus Ptc to other known Ptc proteins. The lineup on which this analysis is based is shown in Fig. S4 in the supplementary material. P. marinus Ptc is positioned midway between the ptc1 and ptc2 clusters of gnathostome vertebrates (note that zebrafish ptc genes were named for the order in which they were cloned, rather than their homology to other known ptc genes). Only regions of ptc sequence from other species that are homologous to our partial P. marinus ptc clone were used for this analysis. zeb, Danio rerio; dros, Drosophila melanogaster; xen, Xenopus laevis; chick, Gallus gallus; mouse, Mus musculus.