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Fig. 2. Early development of the lamprey otic vesicle. (A-I)
Projections of confocal z-stacks of embryos stained with
FITC-phalloidin, revealing the actin-rich stereocilia of the sensory hair
bundles and the general morphology of the developing lamprey inner ear. All
images are of L. fluviatilis, except for A' (L.
planeri) and H,I (zebrafish). (A-I,A') Lateral views, anterior to
the left, dorsal to the top; (C'-G') dorsal views, anterior to the
left, medial to the top. (A-C,E,F) Partially dissected specimens; (D,G)
composites of two images. Arrows indicate outpocketing of endolymphatic duct.
g, statoacoustic (VIIIth) ganglion; ed, endolymphatic duct; hcs, sensory hair
cells. (E,E',I) Double stains with FITC-phalloidin (green) and
anti-acetylated tubulin (red) to reveal neuronal cell bodies and axonal
projections of the statoacoustic ganglion (E,E') and sensory hair cell
kinocilia (I). Stages are shown for each panel. Scale bars: 50 µm.