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Fig. 4. Immunohistochemical analysis. Double immunostaining of CK14 (A,D,G)
and Notch2 (B,E,H) in the epithelial sheaths of mutant incisors grown under
kidney capsules (A-I), and staining for Notch2 in lower incisors of wild-type
mice at PN d1 (J). (A-I) Both labial and lingual HERS are strongly
labeled by anti-CK14 and Notch2 antibodies. Fragmentation of the dental
epithelium (arrowheads), similar to Malassez epithelial rests, is also
recognizable at the dentin surface. Arrows indicate non-specific staining
against calcified dentin. (D-F,G-I) Higher magnifications of the labial HERS
and of the lingual HERS,, respectively, in A-C. (C,F,I) Phase pictures of A,B,
D,E and G,H, respectively. (J) Immunostaining for Notch2 at the apical
end of PN d1 mouse lower incisors. Notch2 is expressed in the OEE and the
stellate reticulum at the labial side (J, smaller arrows), and in the
epithelial sheath at the lingual side (J, larger arrow). Scale bars: 25 µm
in A-C; 10 µm in D-I; 200 µm in J.
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