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Fig. 6. Analysis of semi-thin cross-sections and electron microscopy.
Transverse sections of the apical regions of mutant incisors grown under
kidney capsules (A-C), and electron microscopic observation of a labial
region (crown analog side) at an apical end (D). (E,F)
Transverse section of upper second molar mesio-buccal root of PN w3 mice.
(B,C,F) Higher magnifications of boxes in A and E. (A) This transverse section
is very similar to that showing root (E,F) in a mouse molar. The ring is made
of dentin, and the inside is dental pulp composed of odontoblasts and dental
pulp cells. At the outer surface of dentin, the epithelial cell rests (B,C,
arrowheads) and the invading dental follicle cells were apparent. (D) Electron
microscopic analysis of the labial region of a mutant incisor. Cementum matrix
is deposited at all dentin surfaces. Cementoblast-like cells (asterisk) are
observed at all outer dentin surfaces. These cells have well-developed
organelles and retain thick bundles of collagen fibers for periodontal
ligament (arrows). A fragmented epithelial cell rest is also recognizable (D,
arrowhead). cm, cementum matrix; d, dentin; od, odontoblasts; df, dental
follicle; p, pulp; pl, periodontal ligament. Scale bars: 100 µm in A; 25
µm in B,C; 5 µm in D; 50 µm in E; 10 µm in F.
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