DEV02311 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Hes1 expressionis downregulated in RPCs following Notch1 inactivation. AP activity at E13.5 in Notch1^f/+;α-Cre;Z/AP (A) and Notch1^f/f;α-Cre;Z/AP (C) embryos is detected in the peripheral (p) optic cup, including both the neuronal progenitors and the cells at the tip (t) of the cup that will differentiate to anterior ocular structures of the iris and ciliary body (the central, peripheral and tip of the optic cup are flanked with white arrowheads). (B) Hes1 protein is detected in the normal Notch1^f/+;α-Cre;Z/AP in the ventricular zone of the central (c) and peripheral optic cup and in the non-neuronal tips. (D) In the Notch1^f/f;α-Cre;Z/AP optic cup, downregulation of Hes1 is observed in the peripheral RPCs, while the expression of Hes1 remains intact in the central optic cup, where Notch1 is not mutated, and in the tips of the optic cup, where Notch1 is not normally expressed. le, lens; nr, neruoretina. Scale bar: 80 mm.

• Supplemental Figure 2 -

  Fig. S2. Colocalization of AP⁺ (Notch1^–) and markers for amacrine, bipolar or Müller glia cells is not detected in the peripheral retina of Notch1^f/f;α-Cre;Z/AP mice. Double immunofluorescent analysis with antibodies against AP and syntaxin (amacrine cells, A,D), AP and PKC-α (bipolar cells, B,E) or AP and cyclin D3 (Müller glia, D,F) demonstrates high expression of AP (red) together with these three markers (green) in the α-Cre;ZAP (A-C) but not in the Notch1^f/f;α-Cre;Z/AP (D-F) P15 peripheral retina. gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer; os, outer segments. Scale bar: 100 mm.