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Figure 5


Fig. 5. Notch1- RPC differentiation into precursors of photoreceptors. The expression of factors involved in photoreceptor differentiation Otx2 (A,C,F,H), Crx (B,D,G,I) and Thrß2 (E,J) was analyzed by in situ hybridization on control (A-E) and Notch1f/f;{alpha}-Cre (F-J) eyes. In the control E13.5 (A,B) and E16.5 (C-E) retinas, the expression of Otx2 (A,C), Crx (B,D) and Thrß2 (E) is detected in the outer neuroblastic layer (onb). In the Notch1f/f;{alpha}-Cre retinas, the expression of Otx2 (F,H), Crx (G,I) and Thrß2 (J) is detected in the different layers of the peripheral retina. le, lens; onb, outer neuroblastic layer; RPE, retinal pigmented epithelium. Scale bar: 100 µm.





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