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Fig. 5. Abnormal layer formation of the olfactory bulb in Fez-deficient
mice. (A-T) Coronal sections of the olfactory bulb (A-D,I-T) or
olfactory epithelium (E-H) from E18.5 wild-type (WT) and
Fez-deficient (Fez–/–) embryos were
analyzed by immunohistochemistry with anti-GAP43, anti-OMP, anti-NCAM, or a
combination of anti-reelin and anti-TBX21 antibodies, or by in situ
hybridization with reelin, Dlx1, Gad67 and tyrosine hydroxylase
(Th) probes. Medial is to the right. Immune complexes were visualized
with DAB (A-H), or Alexa488 (anti-reelin) and Alexa568 (anti-TBX21, M,N). In
situ signals were stained with BM purple substrate. GAP43 and OMP were
expressed in OSNs and in the olfactory nerve layer (ONL) in the wild-type
bulb. (A-F) GAP43- and OMP-positive OSNs did not extend after the lamina
cribrosa and formed the FCM in Fez-deficient embryos. The expression
of GAP43 and OMP in the nasal epithelium was not affected in
Fez-deficient embryos. Fez-deficient embryos showed an
aberrantly wide mitral cell layer, which expresses reelin and TBX21 (K-N), and
displayed a reduced number local circuit neurons, which express Gad67
and Th (Q-T), with aberrant positioning. Periglomerular cells and
granule cells are marked by arrowheads and arrows, respectively (Q,S). (O,P)
Progenitors for local circuit neurons were located in the
ventricular/subventricular zone (VZ/SVZ) and expressed Dlx1; the
number of Dlx1-expressing cells was reduced in the olfactory bulb of
Fez-deficient embryos.
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