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Fig. 9. Fez is required in olfactory sensory neurons for axon targeting
and OB layer formation. (A-I) The OMP protein (A-C), and the reelin
(D-F) and Gad67 (G-I) transcripts in control (WT) and
Fez-deficient (Fez–/–) mice, and in
Fez-deficient mice in which Fez expression was rescued under
the control of the olfactory sensory neuron-specific #123 promoter
(Fez–/–, Tg+), at postnatal day 0 (P0) were
detected by immunohistochemistry or in situ hybridization.
Fez+/– mice containing a transgene composed of
#123 promoter-linked Fez-IRES GapVenus expression units
[Fez+/–,Tg(#123p-Fez)] were crossed with
Fez+/– mice to generate control mice (WT),
Fez-deficient mice (Fez–/–) and
rescued Fez-deficient mice (Fez–/–,
Tg+). In some of the rescued Fez-deficient embryos, formation of the
ONL (OMP-positive, C), the mitral/tufted cell layer (MCL, reelin-positive, F),
and the layer of local circuit neurons (glomerular layer, GL; granule cell
layer, GCL) was rescued in one or both bulbs. Three different transgenic
alleles were used. The numbers of rescued and non-rescued embryos are shown in
Table 1. (J)
Transgene-mediated Fez expression in the olfactory epithelium (OE),
but not the olfactory bulbs (OB), of Fez–/–
mice partially rescued axon projection (arrowhead) of OSNs at E14.5. The OSNs
expressing the transgene were monitored by immunohistochemistry with an
anti-GFP (anti-Venus) antibody.
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