spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. eve and grain play additional roles outside of the eve->grn->zfh1 cascade. (A-E) Stage 15 embryo stained for Grn (A,B) ß-Gal (A-D) and Zfh1 (A,C,E). B-E are identical to A but with different combinations of color channels to facilitate the observation of Grn and Zfh1 expression in aCC (arrows) and RP2 (arrowheads). grn is unable to rescue eve mosaic mutants (UAS-grn, eve mosaic; RN2-GAL4, UAS-{tau}lacZ), evident as a failure of aCC and RP2 to project axons out of the VNC, and of aCC to express Zfh1. (F-H) Stage 15 embryo stained for Myc and Zfh1, expressing only UAS-EGFPF (F), UAS-eve (G) or co-misexpressing both eve and grn (H). (F) In the control, dMP2 axons project posteriorly in the longitudinal connective and never exit the VNC laterally (n=62). (G) Ectopic eve triggers lateral VNC exit, but only in 5% of hemisegments. (H) Ectopic eve and grn (UAS-eve, UAS-grn, dMP2-GAL4; UAS-EGFPF) triggers lateral VNC exit in 40% of hemisegment (n=84). There is no evidence of Zfh1 expression in dMP2 neurons (yellow circles), in the control (F) or in the misexpression backgrounds (G). Arrowheads indicate dMP2 axons exiting the VNC.





Right arrow Return to article