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Fig. 5. Effects of the loss of ik2 on microtubule organization in the oocyte. (A,B) Expression of Tau-GFP in wild-type (A) and ik2¹ (B) stage 7-8 egg chambers. An abnormal enrichment of Tau-GFP is seen around the oocyte nucleus in ik2¹ (arrow). Tau-GFP is also seen at higher levels in the nurse cells of the mutant than in wild type. (C,D) Localization of Kinesin-ß-gal, as assayed by antibodies to ß-galactosidase, in wild-type (C) and ik2^alice (D) stage 9 oocytes, shows that the plus-ends of microtubules in ik2^alice show the normal posterior enrichment. (E,F) The localization of dynein heavy chain (DHC) in wild-type (E) and ik2¹ (F) stage 9 oocytes; DHC is seen throughout the lateral cortex of ik2¹ oocytes, in contrast to its posterior localization in wild type. (G-J) Nod-ß-gal as assayed with antibodies to ß-galactosidase in wild-type (G,I) and ik2¹ (H,J) oocytes. In contrast to the dorsal anterior localization of Nod-ß-gal in wild-type stage 8 oocytes (G, arrow), Nod-ß-gal is ectopically localized at the posterior and lateral cortex of the ik2¹ (H) oocyte. By stage 10, Nod-ß-gal was not detected in the ik2¹ oocyte (J), whereas it was localized at the anterior of the wild-type oocyte (I). Nuclei are stained with DAPI (blue). Anterior to the left, dorsal up.

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