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Fig. 3. Actin cytoskeleton organization and 
-tubulin staining and Tau-GFP
localization in wild-type and spn-F ovaries. (A,C,E,G,I)
Wild-type egg chambers; (B,D,F,H,J) spn-F egg chambers.
(A,B) Young egg chambers. -tubulin, red; DNA, blue.
(C,D) Stage 9 egg chambers. -tubulin, red. Abnormal
aggregates of tubulin are seen at the nuclear periphery in the mutant egg
chambers (arrow in D). (E,F) Abnormal accumulation of Tau-GFP is
visible, associated with the mutant oocyte nucleus. Tau-GFP, green; DNA, blue;
nuclear membrane, red. (G,H) Tau-GFP forms a gradient from
anterior to posterior at stage 9 in both wild type and mutant; however, an
abnormal accumulation of Tau-GFP occurs at the oocyte nuclear membrane in the
mutant. Tau-GFP, green; DNA, blue. (I,J) Abnormal clumps of
actin can be seen in the mutant egg chamber (J), compared with wild type (I).
Rhodamine-phalloidin staining, red; DNA, blue.
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