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Fig. 2. Cell proliferation and survival requires msk in the developing
eye. (A-L) Third-instar (A-I) and 60-hour pupal (J-L) eye-imaginal
discs containing clones of msk null cells, revealed by negative
marking using GFP (D,G,J); outlined in (D-I). Anterior is to the right; A-C
and D-L are the same scale as indicated in A and D, respectively. In E,H,K,
antigens are indicated on the left; F,I and L are merged images showing GFP
(green) and antigens (red); A-C shows a time course following the
hsp70-driven induction of clones. Brightly labeled cells are
homozygous wild-type `twin-spots' (white filled arrows), gray cells are
heterozygous and black cells are homozygous msk nulls (black filled
arrows). Note, late clones (48 hours before dissection in A) are numerous,
small, equally distributed on both sides of the furrow, and are accompanied by
twin spots of roughly equal size; earlier clones (72 hours before dissection
in B) are rare, smaller than their twin spots, and are present only posterior
to the furrow. Note also that very early clones (96 hours before dissection in
C) are absent, with only rare, large twin spots remaining. Cell-cycle markers
(BrdU for S-phase (D-F) and Cyclin E for G1 (G-I) are not eliminated by
msk loss of function (arrows). Note, msk null cells
posterior to the furrow eventually die during pupal life, as revealed by
activated Caspase 3 antigen (arrows in J-L).
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