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Figure 3


Fig. 3. msk is not required for cell-type specification in the developing eye but does contribute to ommatidial rotation. (A,D,G,J,M,P) Third-instar eye-imaginal discs containing clones of msk null cells, revealed by negative marking using GFP. (B,E,H,K,N,Q) Antigens visualized by the immunostain indicated at the left. (C,F,I,L,O,R) Merged images, with GFP in green and antigens in red (Elav, blue in R). Anterior is to the right; all images are at the same scale, see bar in P. (A-C) msk loss-of-function does not affect the neural marker Elav (arrows). (D-F) Senseless expression in R8 cells (arrow). (G-I) Spalt expression in R3 and R4 (arrows). (J-L) Cut expression in the cone cells (arrow). (M-R) msk loss-of-function affects ommatidial rotation (M-O), as revealed by BarH1 antigen labelling of R1 and R6 (normal orientation is indicated by an arrowhead and reversed by an arrow), but not chirality (P-R), as revealed by E(Spl)M-delta-0.5:lacZ expression in R4 cells (reversed ommatidia have reversed a R3/4 position, arrow). Note, occasionally two cells are specified as R4 in msk clones (arrowhead in P-R).





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