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Fig. 2. Distribution of Ngn3-positive spermatogonia and
Kit-positive spermatogonia in the P5 testes. (A-D)
Double-staining ISH for Ngn3 (purple, A) and Kit (red, B),
with overlay images of A and B (C), and of B with DNA staining (blue, D).
Purple arrows indicate Ngn3 in spermatogonia; short and long pink and
red arrows indicate Kit in spermatogonia and interstitial cells,
respectively. Note that the Ngn3 and Kit signals are
observed in distinct cells in separate seminiferous tubule cross-sections.
Scale bar: 100 µm. (E-G) ISH for Ngn3 and Kit on
adjacent sections, under low magnification. The distribution of Ngn3
(E) and Kit (F) germ cell signals are compared and represented in G,
where seminiferous tubule cross-sections containing Ngn3 (green),
Kit (blue), or both signals (red) are indicated. For statistical
evaluation see Table S1 in the supplementary material. Scale bars: 100 µm,
in A for A-D, in F for E,F.
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