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Fig. 6. Inhibition of HH signaling enhances epithelial budding. (A,B)
Whole-mount in situ hybridization showing enhanced Ptch1 expression
in the sub-epithelial mesenchyme following incubation with FGF9 (B), and
inhibition of expression when incubated with cyclopamine (C).
(D) Quantified decrease in distal epithelial buds in explants incubated
with FGF9 and increased buds in explants treated with cyclopamine.
(E-G) FGF9 treated E12.5 explants showing epithelial expansion and
reduced branching (F) compared with controls. Cyclopamine-treated explants (G)
exhibit increased epithelial budding (three versus two terminal branches;
arrowheads in E,G). Lung epithelium is labeled with an antibody to TTF1.
Student's t-test, n=2 or 3 explants for each condition;
**P<0.003; *P<0.02.
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