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Figure 1


Fig. 1. Analysis of germ cell loss in Fgf9–/– gonads. (A) Alkaline phosphatase staining for germ cells in Fgf9 mutant gonads at 12.5 dpc. XY Fgf9–/– gonads show a severe reduction in germ cell numbers relative to siblings. (B) Quantification of germ cell loss. Germ cell numbers are similar in mutants and controls from 7.5 to 11.5 dpc (11.5 dpc shown). At 12.5 dpc XY Fgf9–/– gonads contain significantly fewer germ cells than XY Fgf9+/–, XX Fgf9+/– or XX Fgf9–/– (n=14 for each timepoint; P<0.01). Scale bar: 250 µm.





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