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Figure 2


Fig. 2. XY Fgf9–/– gonads show elevated levels of germ cell death between 11.5 and 12.5 dpc. Germ cells are stained for E-Cadherin (blue), whereas laminin (green) labels the basal lamina of testis cords. Cell death is labeled by LysoTracker Red DND-99 (red). XX and XY Fgf9+/– gonads show low basal levels of cell death at both 11.5 dpc (A,G), 12.0 dpc (B,H) and 12.5 dpc (C,I). XX Fgf9–/– gonads contain similar low levels at 11.5 dpc (D), 12.0 dpc (E) and 12.5 dpc (F). XY Fgf9–/– gonads show significantly increased levels of dying germ cells at stages 11.5 to 12.5 dpc (J-L; P<0.05. g, gonad; m, mesonephros. Broken line indicates boundary of gonad and mesonephros. Figures are representative of three independent experiments of n≥3 each. Scale bar: 50 µm.





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