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Figure 3


Fig. 3. Tak1{Delta}/{Delta} embryonic heart phenotype. (A,C,E) Wild-type E9.5 embryos. (B,D,F) E9.5 Tak1{Delta}/{Delta} embryos. (A,B) Sagittal sections immunostained with an antibody to smooth muscle {alpha} actin, labeling the myocardium (brown). Arrowheads indicate trabeculation within each ventricle. Endocardial cushion formation takes place normally in both embryos (arrows) and the curved myocardium in both panels indicates that looping has taken place. (C,D) Embryos labeled with an antisense probe to Gata4. Gata4 labels the extension of the primary heart field and inflow tract at this stage (arrowhead). (E,F) Embryos labeled with an antisense probe to Anf, marking the atrium and ventricle. A, atrium; V, ventricle.





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