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Figure 1


Fig. 1. Mural CD34+ cells in HITA wall. Besides the expected positive staining for CD34 in the endothelial layer (EC), CD34+ cells were found at the transition area from the smooth muscle cell (SMC) to adventitial layer (ADV) (A). Higher magnification reveals the close proximity of these cells to the external elastic membrane (arrows) of the vascular wall (B). FACS analyses on cells isolated from HITA wall after removal of mature endothelial cells positive for CD105 confirm the existence of mural CD34+ cells that are partially positive for von Willebrand Factor (vWF) (C,D). (E,F) Immunostaining for {alpha}-smooth muscle actin revealed a clear staining of smooth muscle cells in the HITA wall (SMC) (E) and in the wall of vasa vasorum (arrows in F) (F) of HITA. No staining is visible in the CD34+ zone of HITA wall, shown at higher magnification in F.





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