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Fig. 2. Downregulation of left Pitx2 expression in
Tbx1-/- embryos at stages E8 and E10. (A-D)
Downregulation of Pitx2 expression in the outflow tract (oft) and in
the left splanchnic mesoderm (sm) is shown in sections cut from whole-mount in
situ hybridized E8 Tbx1-/- embryos (B,D), compared with
wild type (A,C). (E,F) Pitx2 expression levels are not changed
in the most cranial part of the head mesenchyme at this stage.
(G,H) Expression of Nkx2.5 in the heart is also
unchanged in the Tbx1-/- embryos (H) when compared with
the wild type (G). (I-N) Whole-mount in situ hybridization (I-L) and in
situ hybridization in sections (M,N) show reduced Pitx2 expression in
the left atrium (la), the outflow tract (oft) and right ventricle (rv) of
E10.5 Tbx1-/- hearts (K,L). Pitx2 expression in
wild-type hearts is shown in I and J. Absence of Pitx2 in the heart
(arrows) is shown in transverse sections of E10 Tbx1-/-
(N) when compared with the normal expression in Tbx1+/-
(M). (O,P) The pattern and intensity of Pitx2
expression is unchanged in the same embryos in the midgut (mg) and in the
umbilical vein (uv). (Q) Quantitative RT-PCR analysis of Pitx2
mRNA expression level on E10.5 whole hearts from wild type and
Tbx1-/- embryos, P<0.05. The results are
representative of six wild-type and 11 Tbx1-/- embryos.
The error bars indicate the s.d. Gapdh expression level was used for
normalization. da, dorsal aorta; hm, head mesenchyme; lv, left ventricle; ra,
right atrium; nt, notochord. Scale bars: 100 µm in A-H; 300 µm in
M-N.
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