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Figure 6


Fig. 6. Developmental defects at E7 are not rescued by incorporation of normal cells into the epiblast and the visceral endoderm. (A,D) Chimaeras made by injection of normal lacZ-expressing ES cells into blastocysts. Normal NT chimaeras at early-streak (A) and headfold (C) stages. (B) NT chimaera with an abnormal shape; (D) NT chimaera with a `large Exe' phenotype. (E,F) Early-streak stage chimaeras made by injection of normal GFP-expressing ICM cells into NT blastocysts. The chimaera in F displays a `large Exe' phenotype (as well as an abnormal shape), in comparison with the one in E. The nuclei are stained in red by propidium iodide. Cytoplasmic GFP appears green. Scale bars: 100 µm.





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