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Figure 6


Fig. 6. Upregulation of Wnt2, Bmp6, Tnni2 and Jag1 in NICD1-activated hearts at E10.5. (A) RT-PCR analysis was performed using RNA from the ventricle with the AV canal. Expression of Wnt2, Bmp6, Tnni2 and Jag1 is upregulated in NICD1-activated hearts. (B-I') Whole-mount and section in situ hybridization confirmed the upregulation of these genes (B,B',D,D',F,F',H,H', wild-type hearts; C,C',E,E',G,G',I,I', NICD1-activated hearts). The upregulation is also observed in NICD1-activated hearts in a Hesr1-null background (J-M). Abbreviations: LA, left atrium; RA, right atrium; LV, left ventricle; RA, right ventricle.





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