DEV02335 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Expression of JAG during stamen development. (A-C) JAG expression detected by in situ hybridization of an anti-sense probe to wild-type tissue. JAG is expressed in all tissue layers of anthers in stage 4 (A), stage 5 (B) and stage 8 (C) flowers. Scale bars: 50 μm.

• Supplemental Figure 2 -

  Fig. S2. Stamen filaments are unaffected in jag nub mutants. (A,B) Plastic-thin sections of the stamen filament in wild type (A) and jag nub (B). (C,D) SEM images of the filament in wild type (C) and jag nub mutant (D). Scale bars: 50 μm for A,B; 20 μm for C,D.

• Supplemental Figure 3 -

  Fig. S3. Histogenesis of the valves. Plastic-thin sections of gynoecia from stage 7-12 flowers showing the development of the tissue layers. Green bars mark the ena tissue layer and red bars mark the enb tissue layer. Cells in the ena and enb start to be distinguishable in size from cells in other layers around stage 9, with the ena layer cells becoming wider and the enb layer cell becoming narrower. The purple arrow at stage 9 points to the development of a third layer of mesophyll that occurs around stage 9.

• Supplemental Figure 4 -

  Fig. S4. Lignification of the enb layer in jag nub fruit. Cross-sections of stage 17 fruit stained with saffranin O (red) and Alcian Blue (blue). (A) Wild-type fruit develop a continuous layer of lignified enb layer cells that stain red. (B) In jag nub fruit, cells in the enb layer can be enlarged (red arrow) and stain similarly to cells of the mesophyll layers.

• Supplemental Figure 5 -

  Fig. S5. Average cell width in the outer-epidermal layer of the gynoecium. Cells are wider in jag and jag nub mutants compared with wild type; however, there is no significant difference in cell width between jag and jag nub mutants. Error bars indicate s.d.

• Supplemental Figure 6 -

  Fig. S6. The effect of abaxial NUB expression on the timing of trichome development in rosette leaves of the moderate JD148-B line. T2 segregating transgenic plants where scored. Trichome number was counted on the abaxial side of leaves from plants that had just bolted. Four leaves were counted for each leaf class. Trichomes that developed overlying the main vascular bundles where not counted. Error bars indicate s.d.

• Supplemental Figure 7 -

  Fig. S7. Expression of FIL and PHB in petals of FIL::NUB transgenic lines. Expression of FIL and PHB detected by in situ hybridization of an anti-sense probe to wild-type and JD148-A tissue. (A,B) FIL expression marks the abaxial side of the petal. (C,D) PHB expression marks the adaxial side of the petal. (A,C) Wild type petals; (B,D) petals from the FIL::NUB transgenic line JD148-A. Scale bar: 50 μm.