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Fig. 1. NUB expression during lateral organ development.
(A-K) NUB expression detected by in situ hybridization using
an anti-sense probe on wild-type tissue. (A-C) Transverse (A,B) and
longitudinal (C) cross sections of vegetative shoots. NUB expression
starts in leaf primordia during initiation (A) and quickly becomes restricted
to the adaxial side (B). NUB expression is not detected in the shoot
apical meristem (C). (D) NUB expression is not detected in the
inflorescence meristem, early stages of flower meristem development, cryptic
bract or sepal primordia. (D-G) NUB expression in stamen primordia is
apparent just prior to organ initiation, and soon becomes restricted to the
adaxial side after initiation. (E) Stage 5 flower showing NUB
expression throughout early stamen primordia. During late stage 5 (F) and
stage 6 (G), NUB expression becomes restricted to the adaxial side of
stamen primorida. (H) During stage 7, NUB expression becomes
restricted to a group of cells in between the two sets of microsporangia
(black arrowhead). (G) During gynoecium development, NUB is initially
expressed in all cell layers of the valves at stage 6. During stage 7 (H,I),
NUB expression becomes restricted to the adaxial side of the valves
and is maintained there until stage 8 (not shown). (J) During early stage 9,
NUB expression can be detected in ovule primordia. (K) NUB
expression is briefly detected in petal primordia at stage 6. ab, abaxial; ad,
adaxial; cb, cryptic bract; fm, floral meristem; im, inflorescence meristem;
ov, ovule; pe, petal; r, replum; sam, shoot apical meristem; se, sepal; st,
stamen; v, valve. Scale bars: 50 µm.
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