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Fig. 2. The nub-1 insertion allele. (A) Diagram of
the nub-1 T-DNA insertion allele. (B) Expression of
NUB and TUBULIN (TUB) detected by reverse
transcription followed by polymerase chain reaction (RT-PCR), using RNA
isolated from Col-0 and jag nub inflorescence tissue.
(C,D) Phenotypes observed in AP1::NUB-RNAi jag-1
transgenic plants. (C) The valve tissues are often shredded near the apical
region of the gynoecium, resulting in exposure of the ovules. (D)
Microsporangia development is absent in the anther (compare with
Fig. 3D). ov, ovule. Scale
bars: 200 µm in C; 50 µm in D.