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Fig. 3. Flower and anther development in jag nub mutants. SEM
images are of stamens taken from stage 12 flowers. (A-C) Wild type (A),
jag (B) and jag nub (C) flowers. Sepal and petal development
is similar between jag and jag nub mutant flowers; however,
anthers are reduced to stubs of tissue and the distal region of the jag
nub gynoecium is abnormal. (D-F) SEM images of the adaxial side of
anthers from wild-type (D), jag (E) and jag nub (F) stamens.
Four elongated sacs of tissue (microsporangia) are apparent in wild type and
jag mutants. In jag nub mutants, however, a small stub of
tissue develops instead, with epidermal characteristics of the connective in
the apical region and cells of uncertain identity in the basal region.
(G) SEM image of the abaxial side of a wild-type anther revealing the
connective that develops in between the microsporangia. (H,I)
SEM images of epidermal cells taken from the connective region of wild-type
anthers (H) and the connective-like region of jag nub anthers (I).
(J) SEM image of a stamen from a jag nub+/- mutant
showing partial development of the microsporangia. (K-M) Plastic-thin
sections (3 µm) of wild type (K), jag (L) and jag nub (M)
anthers. (K) In wild-type anthers, four locules develop on the adaxial side
separated by the connective on the abaxial side. Black arrowheads indicate
dehiscence zones. (L) In jag mutants, two locules appear to develop
and will split through a dehiscence zone that develops down the middle of the
microsporangia (black arrowheads). (M) In jag nub mutants, no locule
or pollen development is apparent. Inset is the same magnification as K and L.
an, anther; co, connective; fi, filament; lo, locule; ms, microsporangia.
Scale bars: 0.5 mm for A-C; 100 µm for D-G,J; 25 µm for H,I; 50 µm
for K-M.
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