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Fig. 4. Characterizing jag nub patterning defects in the anthers using
FIL, PHB and SPL expression. (A-D) Expression of
FIL detected by in situ hybridization using an anti-sense probe. (A)
During wild-type stamen development, FIL is initially expressed
throughout the abaxial side at stage 6. (B) As the adaxial microsporangia
proliferate, FIL-expressing tissues develop into the connective and
grow more slowly in comparison to the adaxial region. Similar to wild type
(C), FIL expression in stage 6 jag nub stamens (D) is
observed throughout the abaxial half. Although the FIL expression
domain is soon dwarfed by the proliferation of the microsporangia in wild
type, in jag nub anthers, no microsporangia proliferation is apparent
and the FIL expression domain extends towards the adaxial side.
(E,F) Expression of PHB detected by in situ
hybridization using an anti-sense probe. (E) In wild type, PHB
expression is detected in cells marking the dehiscence zone in between the two
microsporangia in each pair (black arrowhead), as well as in the vasculature
(red arrowhead). (F) In jag nub mutants, PHB expression is
only detected in the vasculature. (G-K) SPL expression
monitored using the SPL::GUS reporter. In wild type,
SPL-reporter activity marks two domains in the anther that develop
into the two sets of microsporangia (G, stage 6 shown, white arrowheads) and
is maintained in the anthers until anthesis (H, stage 12 shown). (I) In
jag nub mutants, the SPL reporter is activated in a similar
spatial pattern to wild type. SPL-reporter activity is maintained in
jag nub anthers until about stage 11 (J), but then disappears by
anthesis (K, stage 12 shown). ab, abaxial side; ad, adaxial side; co,
connective; gy, gynoecium; ms, microsporangia. Scale bars: 50 µm.
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