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Fig. 4. Delayed AP1 induction and reduced CAL expression in
lmi1-1 lfy-10. (A) Quantitative real-time RT-PCR analysis
of the expression of the known direct LFY targets AP1, CAL, and
LMI1 through LMI5 in eleven-day-old lmi1-1 lfy-10
compared with lfy-10. The values were normalized using eukaryotic
translation initiation factor 4A (EIF4). Expression levels in
lfy-10 lmi1-1 were set to one to facilitate comparison of the
expression of the different genes. The mean and standard error of three
technical replicates is shown. Similar results were obtained using an
independent biological replicate (not shown). (B) Quantitative
real-time PCR amplification of CAL and AP1. For CAL
expression, lmi1-1 lfy-10, lmi1-4 lfy-10 and lfy-10
seedlings were assayed at 7, 9, 11 or 13 days of age. For AP1
expression, lmi1-1 lfy-10 and lfy-10 seedlings were assayed
at 9, 11 or 13 days of age. Similar results were obtained for both
CAL and AP1 using an independent biological replicate (not
shown). Normalization was as in A. (C) Transverse sections through the
inflorescence meristems of fixed fifteen-day-old lfy-10 (top) and
lmi1-1 lfy-10 (bottom) seedlings. Flowers (F) and secondary
inflorescences (I) are indicated. Scale bars: 100 µm.
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