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Fig. 5. LMI1 binds CAL promoter proximal elements. (A) Map
of the promoter proximal region of LMI1. The pseudo-palindromic class
I HD-ZIP-binding site (CAATNATTG) and the LFY-binding site (CCANTG) are
indicated above the diagram, as is the CAL translation start site
(long arrow). Below the diagram is the region amplified by chromatin
immunoprecipitation by William et al.
(William et al., 2004) and in
B. (B) Quantitative real-time PCR amplification of regulatory regions
upstream of CAL and AP1, before (input) and after chromatin
immunoprecipitation (ChIP). Plants expressing MYC-LMI1 (+) were compared with
those not expressing MYC-LMI1 (-). Shown is the mean±s.e.m. of the ChIP
signal normalized by the input signal. Two technical replicates were
performed. Similar results were obtained using an independent biological
replicate (not shown).
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