DEV02338 Supplementary Material

Files in this Data Supplement:

• Supplemental Table - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Broad low-level expression of Sens in AWM and PWM before the precursors of the mechanosensory and the PWM bristle are selected. (A-A″) Double-staining of an A101-lacZ pupal wing at 2 hours APF for β-Gal (green) and Sens (red). Anterior is to the left. At this stage, the AWM chemosensory pIs are selected and co-express high levels of Sens and β-Gal. Two bands of low-level Sens expression persist along the WM.

• Supplemental Figure 2 -

  Fig. S2. Sens is expressed in PWM bristle precursors. (A-A″) Close-up of the PWM of an 8- to 10-hour APF A101-lacZ pupa stained for β-Gal (green) and Sens (red). Sens expression is refined to single cells in the two rows of PWM precursors (compare with Fig. S1). Sens and β-Gal colocalize in these precursors.

• Supplemental Figure 3 -

  Fig. S3. Overexpression of the anti-apoptotic protein P35 does not rescue the sens mutant phenotype in WM mechanosensory organs. (A) A MARCM sens clone that overexpresses P35. Note that the bristle loss is not rescued. (B,B′) Close-up view of the AWM of a 24- to 30-hour APF pupa with a MARCM sens clone overexpressing P35. Nuclear GFP (blue) marks the mutant tissue. Note that none of the sens mutant cells express Elav (red).

• Supplemental Figure 4 -

  Fig. S4. Overexpression of da is able to induce high levels of Sens in single cells in the absence of the ASC. (A-A′) A MARCM Df(1)sc-B57 clone in the thorax (green) expressing da under the control of Eq-GAL4, stained with anti-Sens (red) and anti-Da (green) antibodies. Da staining identifies the ASC clone. Note that similar to the wild-type tissue, Sens accumulates in single cells, the presumptive pI cells, upon overexpression of da in ASC clones.