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Fig. 4. Da and Sens synergize in vitro and in vivo, and bind to each other.
(A-D) Co-expression of Da and Sens in the wing results in a synergistic
increase in the number of ectopic SOs generated. The third wing vein region of
(A) sca109-68-GAL4/UAS-da52, (B)
sca109-68-GAL4 UAS-sensC12 and (C)
sca109-68-GAL4 UAS-sensC12/UAS-da52
flies are shown. In D, the number of extra SOs are quantified for each
genotype (n
17). Arrows in insets indicate the extra campaniform
(dome-shaped) sensilla. (E) Sens strongly increases the level of
ac-luciferase transcription induced by Da. (F) In
vitro-translated Sens can be pulled-down using GST-Da, but not with GST alone.
Western blotting with an anti-Sens antibody was used for detection.