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Fig. 5. Overexpression of Sens and Da in the thorax can generate bristles in ASC clones. (A) Loss of Ac and Sc leads to a loss of all bristles in the thorax. (B,C) Overexpression of (B) Da or (C) Sens using Eq-GAL4 results in the formation of numerous bristles in a sc^10-1 background. Note that overexpression of Sens in the midline region causes some thorax closure defects and abnormal midline morphology. (D) Overexpression of Sens using a moderate UAS-sens transgene is able to induce a few SOs in a sc^10-1 background. (E) Co-expression of Sens and Da results in the generation of a large number of SOs in sc^10-1 flies. Note that because flies used in D and E were taken out of the pupal case as pharate adults, their cuticle is somewhat wrinkled. (F-H) MARCM clones of Df(1)260-1. Mutant bristles are yellow and singed. In clones of the ASC in the thorax, which are normally devoid of bristles (F), overexpression of (G) Da or (H) Sens induces SO formation.

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