spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Lowering FGF8 levels causes a reduction in placode formation and neural differentiation. (A-E) X. laevis embryos displayed dorsoanteriorly. Embryos were cultured with XlMOF8 (10 ng) or MOSAF8a (60 ng) until the neural tube stage 20; lineage tracer (pink). (B,C) XlMOF8 caused sox2 mispatterning (40/42), so did MOSAF8a but to a lesser degree (20/22). (E) MOSAF8a resulted in a slight expansion of the rx1 domain toward the posterior (16/20). (F-M) The effect of FGF8 reduction on early neuronal differentiation in X. tropicalis. XtMOF8 (8 ng), MOSDF8 (17 ng) and MOSAF8a (16 ng) were injected into one cell at the two-cell stage; injected side is oriented downwards. (F,H,J,L) Embryos were cultured until neurula stages; injected embryos demonstrate an early strong reduction in neuronal differentiation [XtMOF8 (20/20), MOSDF8 (9/9), MOSAF8a (16/16)]. (G,I,K,M) Embryos were cultured until the early tadpole stage; injected embryos demonstrate posterior truncations and continued reduction in differentiated neurons





Right arrow Return to article