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Fig. 9. Analysis of expression directed by the complete ecmA promoter
in control cells, mybE– cells and mybE– cells expressing a
MybE:GFP fusion gene. (A) The ecmAO-lacZ construct and the
pspA gus constructs were co-transformed into Ax2 cells and mybE– cells,
and their spatial expression patterns were determined, by double staining at
the slug stage. (B) Cells transformed as in A were analysed at the slug
stage for lacZ expression alone, i.e. for the activity of the ecmAO promoter
fragment. (C) MybE– cells were co-transformed with a MybE:GFP
fusion construct under the transcriptional control of a semi-consitutive
promoter and with ecmAO-lacZ. The panels on the right show a
phase-contrast image of growing cells juxtaposed with the GFP fluorescence
image of the same field. The panel on the left shows slugs, derived from the
same population, and stained for lacZ expression.
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