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Fig. 3. Expression pattern of intercellular adhesion proteins in ENCCs. Immunolocalisation of intercellular adhesion proteins (in green) on frozen sections of E11.5 distal midgut (A,C,F,H,J) and paraffin sections of E15.5 small intestine (B,D,G,I,K). ENCCs, or neurons and their precursors, are detected with p75^NTR (in red, A,B,J,K) and HuD (in red, C,D,F-I), respectively. (B) N-cad is expressed at the same level by all the neurons and glial cells of the E15.5 myenteric ganglia (p75^NTR+ cells). (D) At E15.5, Cad6 is expressed at high levels by a population of myenteric neurons (HuD⁺ cells, white arrowheads), and at lower levels by other neurons and HuD^- cells, closely associated to neurons, very likely to be glial cells (white arrow). (F-I) Cad11 and NCAM are expressed by both neurons and glial cells (white arrows), and at lower level by the smooth muscles and the submucosal zone. (K) L1-CAM is detected in fibre-like structures in the E15.5 p75^NTR+ myenteric ganglia, likely to be neuronal processes. All these proteins, except L1-CAM, are already expressed by isolated ENCCs located near the migratory front at E11.5 (lines A,C,F,H,J). (E) Deconvolution analysis shows a cell surface localisation for N-cad (in green) and intracytoplasmic accumulation of Cad6 (in red) in E13.5 ENCCs. m, smooth muscles; sz, submucosal zone. Scale bar: 5 µm for A,C,E,F,H,J; 50 µm for B,D,G,I,K.

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