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Fig. 6. The mutant ENCCs show a migration defect in a 3D tissue environment.
(A) Schematic representation of the graft experiment protocol. Segments
of control or mutant distal midgut were grafted onto segments of wild type
hindguts at E12.5. (B,C) X-Gal staining of the explants after 3
days in culture. The black line represents the limit between the wild-type
hindguts and the control or mutant fragments which are grafted onto them. Each
red arrowhead indicates the position of the most-caudal ß-gal+
cell in the wild-type hindgut. Mutant ENCCs migrated less far than control
ENCCs in wild-type hindguts. (D,E) Quantification of the
migration defect. (D) For each of the five independent experiments, noted e1
to e5, the control explant in which ENCCs had migrated the furthest was chosen
as the reference (100). The distances travelled by ENCCs in the other explants
of the same litter were expressed as percentages of this maximal distance. (E)
Histogram showing the average distances covered in wild-type hindguts. The
average distance covered by mutant ENCCs is significantly reduced compared
with the control (*P<0.002).
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