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Fig. 5. Loss of aPKC
results in disappearance of neuroepithelial
adherens junctions. (A-D) Immunofluorescence for aPKC
(red), ß-catenin (green) and nuclei (blue) on confocal sections of the
neocortical region in control (A,C) and aPKC cKO embryos (B,D) at
E15.5. Immunofluorescence of ß-catenin alone is shown in C and D.
Dot-like signals of ß-catenin are constantly seen in control embryos
(A,C; arrowheads), but are rare in aPKC cKO embryos (B,D; arrow).
(E-H) Electron micrographs of the ventricular surface of
neuroepithelium in E15.5 aPKC cKO embryos. (G,H) High-magnification
views. Adherens junctions (electron dense lines indicated by arrowheads) are
constantly observed in the caudal neocortical region where aPKC is
still retained (E,G), while only fragmented adherens junctions (arrow) are
rarely observed in the rostral region where aPKC is predominantly lost
(F,H). LV, lateral ventricle. Scale bars: 10 µm in A,B; 1 µm in E,F.
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