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Figure 2


Fig. 2. Inhibition of NF-AT signaling by DN XNF-ATc3 or CsA does not affect hinge point formation. (A) Phalloidin (red) was used to stain the accumulated apical actin in hingepoints (white arrowhead) of control embryos. White line shows width of the neural tube. (B) Embryos were injected with 2 ng DN XNF-ATc3 and 500 pg EGFP- RNA at the two-cell stage. Although the neural plate appears wider (horizontal line) on the injected side, phalloidin staining (white arrowhead) is not disrupted. Vertical line sketches the midline of the embryo. (C) GFP fluorescence marking the injected side. (D) Transverse section of the embryo in B showing phalloidin staining (arrowheads). (E) GFP fluorescence of D. (F) Phalloidin staining of a control embryo and (G) of an embryo treated with 400 µM CsA at the gastrula stage. Arrowheads in F,G indicate normal phalloidin staining. (H-L) Cell aggregation assay. (H) Uninjected control neural cells dissociated in CMFM. (I) Neural cells injected with 2 ng DN XNF-ATc3 dissociate faster and more completely than controls. (K) The reaggregation of control neural cells in calcium containing medium (1/3 NMR). (L) Only a few DN XNF-ATc3 expressing cells re-aggregate in 1/3 NMR.





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