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Fig. 2. Posterior shift of the IsO in Sp8-/-embryos.
Whole-mount in situ hybridization showing lateral views of E8.5 control
(A) and mutant (B) embryos hybridized with Otx2 and dorsal views
of Gbx2 expression in control (C) and mutant (D) embryos at a
similar stage of gestation. Rhombomeres (r), 3 and 5 are labeled in all
embryos with Krox20 transcripts. The distance between the posterior limit of
Otx2-expression and the anterior boundary of r3 is indicated by a red line in
mutant and control embryos. This line also indicates the distance between the
posterior limit of Gbx2-expression in r1 and the anterior boundary of r3.