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Fig. 3. At E9.5, the expression domains of genes transcribed at the MHB is
enlarged. Wholemount in situ hybridization showing the expression of
Otx2 (A,B), Gbx2 (C,D),
Fgf8 (E,F), En1 (G,H),
Pax2 (J,K) and Wnt1 (L,M) and
transcribed at the MHB, as indicated. At this stage of development (E9.5), the
shift of the IsO, detected at E8.5 and shown in
Fig. 2, is still evident (A-D),
and the expression domains of the presented markers appear enlarged at the MHB
of the mutant embryos (D,F,H,K,M). The embryos shown for Wnt1 are
between E9.5 and E10.
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