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Fig. 4. At E10.5, the Otx2/Gbx2 expression boundary appears normal but
ectopic expression of Fgf8, Otx2 and Wnt1 is found in the
ventral part of the rostral hindbrain. The expression of Otx2, Gbx2,
Wnt1 and Fgf8 is shown on consecutive sagittal sections of E10.5
control and mutant embryos, as indicated. The embryos in B and D are different
from those in F and G. (A-D) Expression of Fgf8 and
Wnt1 is presented in consecutive sagittal sections of wild type (A,C)
and mutant (B,D) embryos. The expression of Fgf8 appears more
affected and therefore more widespread when compared with Wnt1, and
ectopic cell patches seem to reach the most posterior midbrain (arrow in B).
In the ventral midbrain, the expression of Wnt1 (D) is patchy and
more widespread than in the control embryo. (E-H) Expression of
Gbx2 and Otx2 on consecutive sagittal sections of wild type
(E,G) and mutant embryos (F,H). The ectopic expression of Otx2 and
Wnt1 in the rostral hindbrain is found only in the ventral part,
while for Fgf8 this is also true for the dorsal part.
(J,K) Expression of Pitx3, as a marker for midbrain
dopaminergic neurons (Smidt et al.,
2004), on sagittal sections of E12.5 embryos. Ectopic
Pitx3 signal can be found in the ventral part of the rostral
hindbrain of Sp8-deficient embryos, arrowhead in K. (L-O)
Expression of ephrin A5 as a marker for the inferior colliculus
(Donoghue et al., 1996) was not
modified at E11.5 of gestation, as detected on sagittal sections of two mutant
embryos (M,O). Ephrin A5 expression is, however, upregulated in the
ventricular zone of the ventral midbrain of these mutants (M,O, arrows) when
compared with wild type embryos (L,N).
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