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Figure 2


Fig. 2. Temperature-sensitive tsr mutantions. (A) Sequence comparisons of yeast, human and Drosophila cofilin sequences (Lappalainen et al., 1997). The cof1-22 mutation that gave a temperature sensitive phenotype in yeast is in red and denoted above the sequence. This mutation was used to predict and engineer a similar temperature-sensitive mutation in Drosophila, tsr139, that is in red and denoted below the sequence. The position of the tsrV27Q mutation is also indicated. (B) The cross generating tsrV27Q/tsr139; tsr{Delta}96 progeny. The conditional alleles P[w+, tsr139] and P[w+, tsrV27Q] together rescued the lethality caused by the tsr{Delta}96 mutation at the permissive temperature and allowed analysis of PCP defects in different tissues.





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