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Figure 6


Fig. 6. Reduction in Fgf8 expression and signaling in the absence of Shh. RNA in situ hybridization for Fgf8, Spry1 and Wnt1. Analysis was performed on midline sections, as ventral and dorsal isthmus are fused just off the midline in Smo-En1 cko mutants (B, inset). (A-F) Dorsal and most posterior mes, isthmus (Is) and r1 are shown (see Fig. 3G) and are outlined where necessary. The thickness of this region is reduced in E10.5 mutants. (A-D) The Fgf8 and Spry1 domains are severely reduced in Smo-En1 cko mutants (B,D arrows). (E,F) Wnt1 is expanded posteriorly in Smo-En1 cko mutants (arrows). (G-J) Posterior mes, Is and r1 of E12.5 control and Smo-Nes cko mutants. The dorsal Fgf8 and Spry1 domains are slightly reduced in the Smo-Nes cko mutants and Fgf8 is shifted posteriorly (arrows). Scale bars: 25 µm.





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