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Figure 1


Fig. 1. Generation of a conditional allele and verifying the generation of a null allele. Schematic representation of the gene targeting strategy (A). A neo cassette flanked with Frt sites (red triangles) and two loxP sites (yellow triangles) were inserted into the Gli2 locus using homologous recombination. ES cell clones were screened using Southern hybridization of Asp718-digested DNA with a 3' external probe (B). PCR primers floxC and floxD flanking the 3' loxP site (blue arrows, A) were used for genotyping (C). Primers floxA and floxD (blue arrows, A) were used to detect the recombined allele (C). The cerebella from Gli2{Delta}//zfd (D) and Gli2{Delta}/{Delta} (E) embryos resembled the Gli2-null cerebellum, which lacks foliation. Gli2-En1 cko mutants (G) at E16.5 appear similar in size and morphology to wild type (F). By E18.5, a reduction in foliation is observed in mutants (I) when compared with wild type (H).





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