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Fig. 3. Developmental series analysis reveals a delay and premature termination
of foliation. At P0, the wild-type (A) and Gli2-En1 cko
mutant (B) cerebella appear similar in size in whole mount. By P2, a
slight difference in size is observed between wild type (E) and mutant
(F). The mutant cerebellum at P5 (J) is clearly smaller than
that of the wild-type (I). Histological analysis of sagittal sections
at P0, P2, and P5 of wild-type (C,G,K) and
Gli2-En1 cko (D,H,L) cerebella show shallower
fissures, and a thinner EGL in mutants. (M-T) The mutant cerebellum
does not increase in size after P8 (N,P,R,T) compared with the continued
growth observed after P8 in wild-type (M,O,Q,S). The overall anteroposterior
pattern of foliation appears intact, although the number of fissures present
in P16 mutants (T) is comparable to the number present in wild type at P2 (S).
(U-Z) At P0, the mutant EGL (X) is thinner than in wild type (U). By
P5, the wild-type EGL has thickened to more than 10 cell layers (V), whereas
the mutant EGL contains 2-4 cell layers (Y). By P8, the wild-type EGL remains
thick (W), in contrast to the mutant EGL, which has been depleted (Z). Arrows
indicate principle fissures forming cardinal lobes, asterisks indicate
fissures forming lobules and open circles represent fissures forming
sublobules. Broken yellow line indicates pial surface between two lobules;
broken blue line outlines the cerebellum. Scale bar: 250 µm in C,D,G,H; 350
µm in K,L; 500 µm in O,P,S,T.
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