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Figure 5


Fig. 5. Removal of Gli activator further reduces foliation in Gli2-En1 cko cerebella. Histological analysis of sagittal sections reveal that only principle fissures form in the absence of Gli1 and Gli2 (A,B). Marking differentiated neurons with NeuN shows few GCs and a thin IGL in Gli2-En1 cko;Gli1-/- mutants (C). In Smo-Nes cko mutants, only the principle fissures form in medial sections (D) and little foliation occurs in lateral sections (E). This phenotype varies (D, inset). Gli2-Nes cko mutants develop more fissures (arrow, F) than Gli2-En1 cko mutants (see Fig. 4). Scale bar: 500 µm.





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