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Fig. 8. A graded series of Shh signaling levels generates varying degrees of
fissure formation in the cerebellum. (A-L) Schematics of sagittal
sections show the morphology of normal cerebella at different stages as well
as adult mutants. (B) Principle fissures at E18.5 are marked with asterisks.
(G) In the absence of Smo function, no fissures form. (H) Mosaic removal of
Smo allows partial rescue by the remaining wild-type cells, and the
principle fissures form, similar to the pattern present in E18.5 wild-type
cerebella. A similar pattern of fissures forms when only Gli3 is
present. (I) In the absence of Gli2, lobulation progresses slightly
further, such that two additional fissures form (arrows), as in P2 wild-type
cerebella. (J) Mosaic removal of Gli2 allows an additional fissure to
form (arrow). (L) When excess Shh signaling occurs, lobulation progresses
further than normal. Pink indicates the PC layer, and blue represents GCPs and
GCs. Abbreviations: ABL, anterobasal lobe; ADL, anterodorsal lobe; CEL,
central lobe; POL, posterior lobe; INL, inferior lobe; pct, precentral; pcu,
preculminate; icl, intraculminate; pr, primary; dcl, declival; intercrural,
itc; ppy, prepyramidal; se, secondary; uvu, uvular; po, posterolateral).
Figures not drawn to scale. Scale bar: 400 µm in A-C,G-J; 425 µm in D,D;
500 µm in F,K-L.
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