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Fig. 2. Eph and Ephrin are targeted to distinct neuronal compartments.
(A-C) View of approximately five segments of the ventral nerve cord of
an unfixed dissected stage 16 embryo double-labeled with the panaxonal BP102
antibody (A) and Ephrin-Fc (B). Ephrin-Fc binds to axons in a pattern that
essentially overlaps that of BP102. (D,E) Homozygous
Ephx652 embryo double-labeled with the panaxonal anti-HRP
antibody (D) and Ephrin-Fc (E). Homozygous Ephx652 embryos
lack all Ephrin-Fc binding, confirming the specificity of Ephrin-Fc binding
and that Ephx652 is null for Eph function.
(F) Ephrin-Fc binding is unaffected in homozygous
onecutlx122 embryos. (G-I) A
scrt-Gal4/+;UAS-Ephrin:myc/+ embryo double labeled with the panaxonal
anti-HRP antibody (F) and anti-myc antibody(G). scrt-Gal4 drives
expression of Ephrin:myc in all neurons where it is targeted to cell bodies.
Anterior is upwards.